Generator

Part:BBa_K902081

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-25)

4S Desulfurization Pathway Optimization Circuit (PlacI/hpaC/RBS/katGLAA)

This part produces HpaC and KatG-LAA under control of an inducible lacI promoter.

KatG is a catalase, and serves to detoxify hydrogen peroxide. HpaC is an oxidoreductase capable of recycling FMN to FMNH2 while converting NADH to NAD. This part is used in conjuctions with the 4S desulfurization pathway to replace DszD. Doing so improves the rate of desulfurization 7-10x.
File-Ucalgary team sulfur 4s enzyme pathway diagram.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 993
    Illegal BglII site found at 1928
    Illegal BglII site found at 2055
    Illegal BamHI site found at 1845
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 509


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Categories
Parameters
None